Rabbit Anti-P-IκBα (Tyr42)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat IκBα (Tyr-42).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | IκBα (Tyr-42) synthetic peptide (conjugated to KLH) matching residues around tyrosine 42 of human IκBα. This peptide sequence has low homology to other IκB proteins, but does have some homology to unrelated proteins that may have a conserved tyrosine phosphorylation motif. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | NFKBIA |
| Molecular Weight: | 38 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IκBα (Tyr-42) peptide (without carrier). The antibody detects a 38 kDa* protein on Western blots of A431 and Jurkat cells treated with pervanadate, but not in control cells. Due to homologies to tyrosine sites on other proteins, it is recommended that the antibody be used to detect phosphorylation of immunoprecipitated IκBα. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; IP 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunoprecipitation
- Cancer research