Rabbit Anti-P-HSP70 (Ser153)
Rabbit polyclonal antibody to human Hsp70 (Ser153), antigen affinity purified from pooled serum.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide from residues around Thr153 of human HSP, conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Human |
| Species Reactivity: | Human |
| Gene Name: | HSPA1A |
| Molecular Weight: | 70 kDa |
| RRID: | AB_2636998 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol |
| Specificity: | Recognizes ~70 kDa Hsp70 phosphorylated at Ser153. Immunolabeling of Hsp70 is completely eliminated with λ-phosphatase treatment. Additional bands detected are thought to be other members of the Hsp70 family. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot