Rabbit Anti-P-Fascin (Ser39)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse and rat Fascin (Ser-39).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Fascin (Ser-39) synthetic peptide (conjugated to carrier protein) corresponds to residues around serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3. |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Human, Mouse, Rat |
| Gene Name: | FSCN1 |
| Molecular Weight: | 55 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to a phospho-serine peptide before affinity purified using phosho-fascin (Ser-39) peptide (without carrier). The antibody detects a 55 kDa* band matching fascin on Western blots of mouse C2C12 and human HeLa cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment. The antibody also detects fascin (Ser-39) phosphorylation in chick DRG neurons. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research