Rabbit Anti-P-Estrogen Receptor α (Tyr537)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus Estrogen Receptor α (Tyr-537).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-ERα (Tyr-537) synthetic peptide (conjugated to carrier protein) corresponds to residues around Tyr-537 in human ERα. The sequence is conserved in rat and mouse ERα, and is also well conserved in ERβ (Tyr-488). |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Frog, Human, Mouse, Rat |
| Gene Name: | ESR1 |
| Molecular Weight: | 35-66 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to unphosphorylated ERα (Tyr-537) peptide before affinity purification using phospho-ERα (Tyr-537) peptide (without carrier). This antibody detects several forms of ERα ranging from 66 to 35 kDa* on Western blots of MCF-7 cells treated with pervanadate, and this reactivity is removed after alkaline phosphatase treatment. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research