Rabbit Anti-P-ERK1/ERK2 (Thr207/Thr188)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus ERK1(T207)/ERK2(T188).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-ERK2 (Thr-188) synthetic peptide (conjugated to carrier protein) corresponds to residues around Thr-188 in mouse ERK2. This sequence is conserved in human, rat, chicken, and fish ERK2, and is highly conserved in ERK1 (Thr-207), ERK5 (Thr-224), and ERK7 (Thr-180). |
| Immunogen Species: | Mouse |
| Species Reactivity: | Chicken, Frog, Human, Mouse, Rat |
| Gene Name: | MAPK1/2 |
| Molecular Weight: | 42/44 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects 42 and 44 kDa* proteins matching ERK1 (Thr-207) and ERK2 (Thr-188) on Western blots of human A431 epithelial cells stimulated with calyculin A. It does not detect these ERK proteins in control cells or in blots treated with lambda phosphatase. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research