Rabbit Anti-P-DOK1 (Ser450)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat Dok1 (Ser-450).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Clone: | WB, E |
| Isotype: | IgG |
| Immunogen: | Phospho-Dok1 (Ser-450) synthetic peptide (conjugated to KLH) corresponds to residues around serine 450 in human Dok1. This sequence is conserved in Dok1 from rat (Ser-449) and mouse (Ser-451). The site is not conserved in other Dok family members. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | DOK1 |
| Molecular Weight: | 62 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to an unphosphorylated Dok1 (Ser-450) peptide before affinity purification using phospho-Dok1 (Ser-450) peptide. The purified antibody detects a band at 62 kDa* matching Dok1 in western blots of human Jurkat cells, but does not detect this band after lambda phosphatase treatment. Similar to Dok1 (Tyr-362) antibody, this antibody also detects an 80 kDa band that has not been identified. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research