Rabbit Anti-P-CRK II (Tyr251)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus Crk II (Tyr-251).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-Crk (Tyr-251) synthetic peptide (conjugated to KLH) matching residues around Tyr-251 in human Crk II. This peptide sequence is well conserved in mouse and rat Crk II, as well as in CrkL (Tyr-251). The site is not found in Crk I. |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Frog, Human, Mouse, Rat |
| Gene Name: | CRK |
| Molecular Weight: | 38 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 38 and 40 kDa* proteins matching the molecular mass of Crk II and CrkL on Western blots of human K562 cells stimulated with pervanadate and A431 cells stimulated with EGF. This reactivity is not observed after akaline phosphatase treatment. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Cancer research