Rabbit Anti-P-γ-Catenin (Tyr550)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat γ-Catenin (Tyr-550).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-γ-Catenin (Tyr-550) synthetic peptide (conjugated to KLH) matching residues around tyrosine 550 of human γ-Catenin. This peptide sequence is highly conserved in rat and mouse γ-Catenin. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | JUP |
| Molecular Weight: | 84 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to both phospho-tyrosine coupled to agarose and to dephosphorylated γ-Catenin (Tyr-550) peptide before affinity purification using phospho-γ-Catenin (Tyr-550) peptide (without carrier). The antibody detects an 84kDa* protein matching the molecular mass of γ-Catenin on Western blots of A431 and Hct116 src transformed cells treated with pervanadate (1 mM for 30 min), but not in control cells. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:50. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research