Rabbit Anti-P-Beta-Catenin (Tyr142)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat β-Catenin (Tyr-142)[γ-Catenin (Tyr-133)].
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-β-Catenin (Tyr-142) synthetic peptide (conjugated to KLH) matching residues around tyrosine 142 of human β-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human γ-Catenin. These human sequences are highly conserved in rat and mouse β- and γ-Catenins. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | CTNNB1 |
| Molecular Weight: | 92 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-β-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein matching the molecular mass of β-Catenin on Western blots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells. |
| Application Notes: | Recommended starting dilutions: WB 1:500; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research