Rabbit Anti-P-CaM Kinase II (Thr306)
Rabbit polyclonal antibody to mouse and rat CaM Kinase II (Thr306), antigen affinity purified from pooled serum.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide from residues around Thr306 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Rat |
| Species Reactivity: | Mouse, Rat |
| Gene Name: | CAMK2A/B |
| Molecular Weight: | 50/60 kDa |
| RRID: | AB_2560940 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol |
| Specificity: | Recognizes endogenous ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with λ-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; WB Brain 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research