Rabbit Anti-P-B-Raf (Ser446)
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat B-Raf (S446)[C-Raf (S338)/A-Raf (S299).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426
Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-B-Raf (Ser-446) synthetic peptide (conjugated to KLH) corresponds to residues around serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299). |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | BRAF |
| Molecular Weight: | 95/105 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A. The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Cancer research