Rabbit Anti-P-Argonaute 2 (Tyr393)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus Argonaute 2 (Tyr-393).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-Ago2 (Tyr-393) synthetic peptide (conjugated to KLH) matching residues around tyrosine 393 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2. The site is also conserved in Ago1 and Ago4, but not in Ago3. |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Frog, Human, Mouse, Rat |
| Gene Name: | AGO2 |
| Molecular Weight: | 97 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-absorbed to unphospho-Ago2 (Tyr-393), before affinity purification using phospho-Ago2 (Tyr-393) peptide. The antibody detects a 97 kDa* protein matching the apparent molecular mass of phosphorylated Ago2 on Western blots of human A431 cells that were treated with EGF. This reactivity is not observed after lambda phosphatase dephosphorylation. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:50. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research