Rabbit Anti-P-α6-Tubulin (Ser165)
Antigen affinity purified rabbit polyclonal antibody to chicken, human, mouse, rat and Xenopus α6-Tubulin (Ser-165).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Phospho-α6-Tubulin (Ser-165) synthetic peptide (conjugated to KLH) corresponds to residues around serine 165 of human α6-Tubulin. The sequence is conserved in most α-tubulin isoforms from most eukaryotic species, but is not conserved in β-Tubulin. |
| Immunogen Species: | Human |
| Species Reactivity: | Chicken, Frog, Human, Mouse, Rat |
| Gene Name: | TUBA1C |
| Molecular Weight: | 50 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to unphosphorylated α6-Tubulin (a.a. 160-169) peptide before affinity purification using α6-Tubulin (Ser-165) peptide (without carrier). The antibody detects a 50 kDa* protein matching the molecular mass of α-Tubulin on Western blots of recombinant a1-tubulin phosphorylated with PKCα. This reactivity is not observed after lambda phosphatase treatment. |
| Application Notes: | Recommended starting dilution: WB 1:1000. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot