Rabbit Anti-P-14-3-3 (Ser58)
Rabbit polyclonal antibody to human, mouse and rat 14-3-3 (Ser58), antigen affinity purified from pooled serum.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide from residues around Ser58 of rat 14-3-3, conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rat, Salamander |
| Gene Name: | YWHAB |
| Molecular Weight: | 29 kDa |
| RRID: | AB_2492027 |
| Purification: | Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns |
| Physical State: | Liquid |
| Buffer: | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol |
| Specificity: | Recognizes endogenous ~29 kDa 14-3-3 protein phosphorylated at Ser58. Labeling is abolished by pre-adsorption with the phosphopeptide used as antigen, and not by the matching non-phosphopeptide. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; WB Brain 1:1000; IHC 1:100 – 1:500. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunohistochemistry
- Cancer research