Rabbit Anti-Neuropilin-1 (a1 CUB Domain)

Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat Neuropilin-1 (a1 CUB Domain).

Rabbit Anti-Neuropilin-1 (a1 CUB Domain)
Rabbit Anti-Neuropilin-1 (a1 CUB Domain)
Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (lanes 1 & 2) or with anti-HA (lanes 3 & 4).
Western blot image of COS-7 cells untransfected (lanes 1 & 3) or transfected with HA-tagged mouse neuropilin-1 (lanes 2 & 4). Blots were probed with anti-Neuropilin-1 (lanes 1 & 2) or with anti-HA (lanes 3 & 4).
Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide in the presence of the anti-NRP-1 antibody (C,F).
Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide in the presence of the anti-NRP-1 antibody (C,F).

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBNR1-100UL

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Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Neuropilin-1 synthetic peptide (conjugated to carrier protein) matching residues within the a1 CUB domain of human neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: NRP1
Molecular Weight: 130
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 130 kDa* protein matching the apparent molecular mass of neuropilin-1 on Western blots of COS-7 cells transfected with mouse neuropilin-1. In immunocytochemistry, this antibody co-localizes with anti-HA in Cos-7 cells transfected with HA-tagged mouse neuropilin-1.
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:100; ICC 1:200.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis