Rabbit Anti-Neurofilament Heavy, Phosphorylated and Non-Phosphorylated (pNF-H)
Affinity purified rabbit polyclonal antibody raised against Neurofilament Heavy, Phosphorylated and Non-Phosphorylated (pNF-H).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized with sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | Mixed |
| Immunogen: | This antibody has been made against a rat NF-H construct containing most of the tandem KSP repeats expressed in and purified from E.coli. |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Rat |
| Molecular Weight: | 160 kDa |
| Purification: | Whole serum (unpurified antiserum) |
| Physical State: | Liquid |
| Buffer: | Lyophilized with sodium azide |
| Specificity: | Species cross-reactivity includes human and rat. This antibody reacts with with phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa. Predicted to react with other mammals due to sequence homology. |
| Application Notes: | Recommended starting dilutions: WB 1:5000 – 1:10000; ICC 1:500 – 1:1000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Neuroscience research