Rabbit Anti-NCC (Thiazide Sensitive NaCl Cotransporter, Thr53)

Rabbit polyclonal antibody to human, mouse and rat NCC (Thiazide sensitive NaCl cotransporter), antigen affinity purified from pooled serum.

Rabbit Anti-NCC (Thiazide sensitive NaCl cotransporter, Thr53)
Rabbit Anti-NCC (Thiazide sensitive NaCl cotransporter, Thr53)
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (red, 1:100,000) on the left and the absence of staining in the KO on the right. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
Two Western blots show the abundance of pNCCT53 (1:200) and tNCC in male Kcnj10fl/fl mice and in Ks-Kir4.1–KO mice treated with vehicle (control) and FK506 (0.75 mg/kg BW). Image from publication CC-BY-4.0. PMID: 36821372
Two Western blots show the abundance of pNCCT53 (1:200) and tNCC in male Kcnj10fl/fl mice and in Ks-Kir4.1–KO mice treated with vehicle (control) and FK506 (0.75 mg/kg BW). Image from publication CC-BY-4.0. PMID: 36821372
Immunostaining of mouse kidney sections from control (CTL) and BRL37344 treated (BRL, 1 mg/kg) specifically labeling localized phosphorylated NCC thr53 (green) and counterstained with Evans Blue (in red). Image from publication. CC-BY-4.0, PMID: 38652212
Immunostaining of mouse kidney sections from control (CTL) and BRL37344 treated (BRL, 1 mg/kg) specifically labeling localized phosphorylated NCC thr53 (green) and counterstained with Evans Blue (in red). Image from publication. CC-BY-4.0, PMID: 38652212
Immunolabeling of mouse kidney paraffin section specifically detecting NCC Thr53 (red, 1:1000) phosphorylation with the increased NCC activation in Ang-II-infused Prdx5 KO kidney. Image from publication, CC-BY-4.0. PMID: 39857434.
Immunolabeling of mouse kidney paraffin section specifically detecting NCC Thr53 (red, 1:1000) phosphorylation with the increased NCC activation in Ang-II-infused Prdx5 KO kidney. Image from publication, CC-BY-4.0. PMID: 39857434.
Western blot image of kidney measuring levels of pNCC-T53 and total NCC in Kir4.2+/+ and Kir4.2−/− mice. Image from publication, CC-BY-4.0. PMID: 38886379.
Western blot image of kidney measuring levels of pNCC-T53 and total NCC in Kir4.2+/+ and Kir4.2−/− mice. Image from publication, CC-BY-4.0. PMID: 38886379.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
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Product code: ARBNCC

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Unit priceFrom $164.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic phospho-peptide from residues around Thr53 of mouse NCC, conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Species: Mouse
Species Reactivity: Mouse
Gene Name: SLC12A3
Molecular Weight: 160 kDa
RRID: AB_2650477
Purification: Antigen affinity-purified from pooled serum on sequential phospho- and non-phosphopeptide affinity columns
Physical State: Liquid
Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Specificity: Recognizes endogenous ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.
Application Notes: Recommended starting dilutions: WB 1:1000 – 1:6000; IHC 1:100 – 1:10,000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Cancer research
Documents & Datasheets

Certificate of Analysis