Rabbit Anti-BetaIII-Tubulin
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat unphosphorylated βIII-Tubulin (Ser-444).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Unphosphorylated βIII-Tubulin synthetic peptide (conjugated to KLH) matching residues around serine 444 of human βIII-Tubulin. This sequence is not found in bI or βII-Tubulin isotypes, but is well conserved in βIII-Tubulins from rat and mouse. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | TUBB3 |
| Molecular Weight: | 50 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | The antibody was cross-adsorbed to phospho-βIII-Tubulin (Ser-444) peptide before affinity purification using unphosphorylated βIII-Tubulin (Ser-444) peptide (without carrier). The antibody detects a 50 kDa* protein matching the molecular mass of unphosphorylated βIII-Tubulin on Western blots of purified brain tubulin and mouse brain tissue. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)