Rabbit Anti-Histone H2B C-Terminus
Antigen affinity purified rabbit polyclonal antibody to human, mouse, rat and Xenopus Histone H2B (C-terminus).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Histone H2B synthetic peptide (conjugated to carrier protein) corresponds to residues in the C-terminal region of human histone H2B. The sequence is conserved in rat, mouse, fish, and drosophila histone H2B, and has low homology to other histone family members. |
| Immunogen Species: | Human |
| Species Reactivity: | Frog, Human, Mouse, Rat |
| Gene Name: | H2BC3 |
| Molecular Weight: | 15 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects a 15 kDa* protein matching the molecular mass of Histone H2B on Western blots of human A431, Jurkat, HeLa, mouse C2C12, rat PC12, and A7r5 cells. The antibody also shows nuclear labeling in immunocytochemistry of methanol/acetone fixed cells. |
| Application Notes: | Recommended starting dilutions: WB 1:5000; ICC 1:200. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research