Rabbit Anti-Growth Associated Protein 43 (GAP-43)
Affinity purified rabbit polyclonal antibody raised against Growth Associated Protein 43 (GAP-43).
Specifications
Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG.
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein. |
| Immunogen Species: | Rat |
| Species Reactivity: | Bovine, Human, Mouse, Rat |
| Molecular Weight: | ~43-57 kDa |
| Purification: | Affinity-purified |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | Reacts with a 43 kDa band by Western blot on bovine cerebellum homogenate. The molecular weight of the protein recognized can vary (~43-57 kDa) depending on the species and the percentage acrylamide used in the SDS-PAGE gel. It has also been used successfully for immunocytochemistry. |
| Application Notes: | Recommended starting dilutions: WB 1:5000 – 1:20000; ICC 1:500 – 1:2000. |
| Storage Conditions: | -20°C or -80°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Neuroscience research