Rabbit Anti-Collagen 1, Alpha 1 Telopeptide

Antigen affinity purified rabbit polyclonal antibody to human, mouse, rat and sheep Collagen 1, alpha 1 telopeptide.

Rabbit Anti-Collagen 1, Alpha 1 Telopeptide
Rabbit Anti-Collagen 1, Alpha 1 Telopeptide
Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.
Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.
Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
Immunofluorescence of a transversal section from mouse spinal cord labeling fibers of collagen (red, 1:200) from the tissue scaffold. The blue is DAPI counterstaining nuclear DNA. Image kindly provided by Dr. Rodolfo Gatto and Dr. Gerardo Morfini, Department of Anatomy and Cell biology, University of Illinois at Chicago.
Western blot of rat lung lysate showing specific immunolabeling of the ~140 kDa collagen protein.
Western blot of rat lung lysate showing specific immunolabeling of the ~140 kDa collagen protein.
Immunolabeling of a hiPSCs seeded sponge section cultured for 28 days labeling COL1A1 1:100, green) expression and nuclei are labeled with DAPI. Image from publication, CC-BY-4.0. PMID: 39664802.
Immunolabeling of a hiPSCs seeded sponge section cultured for 28 days labeling COL1A1 1:100, green) expression and nuclei are labeled with DAPI. Image from publication, CC-BY-4.0. PMID: 39664802.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS
Learn more

Product code: ARBCAPV2

Custom/Bulk request
Unit priceFrom $164.00
Add to Quote

Lead time on this item is currently: 2-4 Weeks

Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426

Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic peptide from residues specific to the C-terminal region of human collagen I alpha 1 telopeptide, conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat, Sheep
Gene Name: COL1A1
Molecular Weight: 140 kDa
RRID: AB_2492061
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS
Specificity: Recognizes endogenous ~140 kDa collagen I alpha-1 telopeptide portion of the collagen I alpha-1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0) / 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:100 – 1:200.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
Documents & Datasheets

Certificate of Analysis