Rabbit Anti-Collagen 1, Alpha 1 Propeptide
Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat Collagen 1, alpha 1 propeptide.
Specifications
Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Learn more PBS
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide from residues specific to the human collagen 1, alpha 1 propeptide conjugated to keyhole limpet hemocyanin (KLH). |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | COL1A1 |
| Molecular Weight: | 180 kDa |
| RRID: | AB_2492060 |
| Purification: | Antigen affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS |
| Specificity: | Recognizes endogenous propeptide portion of the ~180 kDa collagen I alpha 1 polypeptide in human lung fibroblast extract. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, the antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; IHC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C or -80°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunohistochemistry