Rabbit Anti-Choline O-Acetyltransferase (ChAT)
Affinity purified rabbit polyclonal antibody raised against Choline O-Acetyltransferase (ChAT).
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | Mixed |
| Immunogen: | A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH. |
| Immunogen Species: | Pig |
| Species Reactivity: | Guinea Pig, Mouse, Pig, Rabbit, Rat |
| Purification: | Whole serum (unpurified antiserum) |
| Physical State: | Liquid |
| Specificity: | This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit. |
| Application Notes: | Recommended starting dilutions: IHC 1:400 – 1:2000; ICC 1:400 – 1:2000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- ELISA
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research