Rabbit Anti-Atrogin-1

Antigen affinity purified rabbit polyclonal antibody to human, mouse and rat Atrogin-1.

Rabbit Anti-Atrogin-1
Rabbit Anti-Atrogin-1
Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide.
Western blot image of mouse gastrocnemius (lanes 1 & 3) and mouse diaphragm tissue lysate (lanes 2 & 4). The blot was probed with anti-Atrogin-1 (lanes 1-4) in the presence (lanes 3 & 4) or absence (lanes 1 & 2) of Atrogin-1 peptide.
Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Formalin fixed, citric acid treated paraffin sections of E16 mouse skeletal muscle. Sections were probed with anti-Atrogin-1 then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
Western blot analysis of MuRF1, Atrogin-1, FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907
Western blot analysis of MuRF1, Atrogin-1, FoxO3a, p-AKT (Ser473), and total AKT in the soleus muscle of control, HU, and HU+TUDCA mice. Image from publication CC-BY-4.0. PMID: 37525907
Western blotting analysis of the expression of Atrogin-1 (1:1000) and MuRF1 (1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807
Western blotting analysis of the expression of Atrogin-1 (1:1000) and MuRF1 (1:1000) proteins in the soleus muscles after HS. Image from publication CC-BY-4.0. PMID: 37344807
Western blot showing MuRF-1 (1:500) and atrogin-1, 1:500) levels in C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400
Western blot showing MuRF-1 (1:500) and atrogin-1, 1:500) levels in C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. GAPDH was used as a loading control. Image from publication CC-BY-4.0. PMID: 37237400
The individual and combined effect of quercetin (Q) and low dose (LL) or high dose (HL) of leucine on relative protein expression of p-FoxO1/FoxO1, Atrogin-1, and MuRF1 in the gastrocnemius muscle in BALB/c mice exposed to cisplatin (CDDP). Image from publication CC-BY-4.0. PMID: 37699022
The individual and combined effect of quercetin (Q) and low dose (LL) or high dose (HL) of leucine on relative protein expression of p-FoxO1/FoxO1, Atrogin-1, and MuRF1 in the gastrocnemius muscle in BALB/c mice exposed to cisplatin (CDDP). Image from publication CC-BY-4.0. PMID: 37699022
Representative Western blots for Atrogin-1 (1:1000) in rat ASM cells that were infected for 24 h with Ad-GFP, Ad-Smad3, Ad-FoxO3, or Ad-Smad3/Ad-FoxO3. Image from publication CC-BY-4.0. PMID: 37693008
Representative Western blots for Atrogin-1 (1:1000) in rat ASM cells that were infected for 24 h with Ad-GFP, Ad-Smad3, Ad-FoxO3, or Ad-Smad3/Ad-FoxO3. Image from publication CC-BY-4.0. PMID: 37693008

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: ARBF32-100UL

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Unit price$348.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Immunogen: Synthetic peptide from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32), conjugated to carrier protein.
Immunogen Species: Mouse
Species Reactivity: Human, Mouse, Rat
Gene Name: Fbxo32
Molecular Weight: 41
Purification: Antigen affinity-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 41 kDa* protein matching the apparent molecular mass of Atrogin on Western blots of mouse muscle tissue lysates. Similar results were seen in C2C12 cell lysate and the band observed in western blot is specifically blocked by Atrogin-1 peptide (AX2045).
Application Notes: Recommended starting dilutions: WB 1:1000; IHC 1:100.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis