Rabbit Anti-Adenylate Cyclase Type III (ACIII)
Affinity purified rabbit polyclonal antibody raised against Adenylate Cyclase Type III (ACIII).
Specifications
Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG.
| Host Species: | Rabbit |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Immunogen: | 20 amino acid peptide identical to the C-terminal region of rat ACIII (residues PAAFPNGSSVTLPHQVVDNP). |
| Immunogen Species: | Rat |
| Species Reactivity: | Human, Mouse, Rat |
| Molecular Weight: | 200 kDa |
| Purification: | Affinity-purified |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | The antibody stains a band at about 200 kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160 kDa. It has also been used successfully for immunocytochemistry on mixed neuron/glia cultures. The antibody has been directly tested for reactivity in rat. It can be expected that it will work on human due to homology with the immunogen and possibly other mammal tissues (human, horse, cow, pig, chicken, mouse). |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:2000; IHC 1:5000 – 1:10000; ICC 1:5000 – 1:10000. |
| Storage Conditions: | -20°C or -80°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research