Mouse Anti-Vimentin (Clone 2A52)

Antibodies to vimentin are useful in studies of stem cells and generally to reveal the intermediate filament cytoskeleton. The immunogen used to generate this antibody was full length recombinant human vimentin, PROT-r-Vim, expressed in and purified from E. coli. The same immunogen was used to produce another monoclonal antibody to vimentin MCA-2D1. Both monoclonal antibodies bind to a region in the C-terminal "tail" region of vimentin included in the peptide SRISLPLPNFSSLNLRE, which is conserved in rat, cow, pig and most other species. Interestingly mouse has the peptide SRISLPLPTFSSLNLRE, differing by just one amino acid, a threonine substituted for a asparagine. As a result neither MCA-2A52 nor MCA-2D1 bind this peptide. These antibodies can therefore be used to identify human or rat cells in a background of mouse cultures or tissues. These two antibodies bind vimentin expressed in a wide variety of species including mouse, rat and human.