Mouse Anti-Beta-Tubulin (Clone 1B12)

β-tubulin is regarded as a “house keeping” protein which is generally not altered much in expression as a result of experimental manipulations. As a result antibodies to β-tubulin are widely used as loading controls in western blotting experiments as a standard by which the levels of other proteins may be measured. As shown here, MCA-1B12 produces a single clean and strong clean band on homogenates of cell and tissue extracts. It will also produce beautiful images of the microtubular network of cells grown in culture and also tissues for ICC but is not recommended for IHC. MCA-1B12 is an IgG2b class antibody and an alternate antibody useful for certain kinds of experiment including IHC is MCA-4E4, an antibody of similar β-tubulin specificity but which is a IgG2a class antibody.