Mouse Anti-Alpha-Tubulin C-Terminus

Protein A purified mouse monoclonal antibody to human, mouse and rat α-Tubulin (C-terminus).

Mouse Anti-α-Tubulin C-Terminus
Mouse Anti-α-Tubulin C-Terminus
Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.
Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.
Immunocytochemical labeling of α- and βI-Tubulin in rat A7r5 cells. The cells were labeled with anti-βI-Tubulin (left) and anti-α-tubulin (right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 488.
Immunocytochemical labeling of α- and βI-Tubulin in rat A7r5 cells. The cells were labeled with anti-βI-Tubulin (left) and anti-α-tubulin (right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 488.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSAL6G1-100UL

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Unit price$292.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: DM1A
Isotype: IgG1
Immunogen: Clone DM1A was generated from purified chick brain tubulin. The antibody recognizes an epitope within the C-terminal end of α-tubulin isoforms. The epitope is highly conserved in α-tubulin isoforms from most vertebrate species.
Immunogen Species: Chicken
Species Reactivity: Human, Mouse, Rat
Gene Name: TUBA1
Molecular Weight: 55
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 55 kDa* protein matching the molecular mass of α-Tubulin on Western blots of human, rat, and mouse cells and tissues.
Application Notes: Recommended starting dilutions: WB 1:500; ICC 1:200.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
Documents & Datasheets

Certificate of Analysis