Chicken Anti-Beta-Tubulin 3 (TUJ)

Three different antipeptide antibodies were generated in chickens against sequences shared between the rat (AAM28438) and human (AAL28094) gene products.

Chicken Anti-Beta-Tubulin 3 (TUJ)
Chicken Anti-Beta-Tubulin 3 (TUJ)
Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr.
Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-GFAP, blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG. Image from Dr.
Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).
Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green, 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).
Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).
Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (green 1:1000). Red staining is rabbit-anti-actin (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY 1:1000); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).
Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000 dilution). Red staining is rabbit-anti-GFAP (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG Dr.
Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (green, 1:1000 dilution). Red staining is rabbit-anti-GFAP (1:1000); blue is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG Dr.
β-Tubulin 3 Immunoblot using homogenates of various organs. Performed at Kingfisher Labs.
β-Tubulin 3 Immunoblot using homogenates of various organs. Performed at Kingfisher Labs.
β-Tubulin 3 Immunoblot using homogenates of adult mouse brain (1:3000).
β-Tubulin 3 Immunoblot using homogenates of adult mouse brain (1:3000).
Representative maximum projection fluorescence images of hNPCs encapsulated at 3 × 104 cells/μl within all three gel formulations after 7 days of culture with labeled cell nuclei (DAPI, magenta) and neuritic extensions (TUBB3, 1:1000; cyan). Image from publication CC-BY-4.0. PMID:37862423
Representative maximum projection fluorescence images of hNPCs encapsulated at 3 × 104 cells/μl within all three gel formulations after 7 days of culture with labeled cell nuclei (DAPI, magenta) and neuritic extensions (TUBB3, 1:1000; cyan). Image from publication CC-BY-4.0. PMID:37862423

Specifications

Physical State:
Liquid
Storage:
2 – 8°C
Buffer:
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative

Release criteria

Protein Concentration:
300 µg/mL
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Product code: ACKTUBIY

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Product Overview

Antibodies were affinity-purified and mixed in equal concentration. These antibodies have been validated with human, mouse and rat tissues.

Host Species: Chicken
Clonality: Polyclonal
Isotype: IgY
Immunogen: Synthetic peptides
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: TUBB3 TUBB4
Molecular Weight: 50 kDa
RRID: AB_2313564
Purification: Affinity-Purified IgY
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative
Protein Concentration: 300 µg/mL
Application Notes: Recommended starting dilutions: WB 1:10000 – 1:20000; IHC 1:2000 – 1:5000; ICC 1:2000 – 1:5000.
Testing: Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage Conditions: 2 – 8°C
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles.
Shipping Conditions: Dry ice
Expiration: Stable for 12 months from receipt.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
Documents & Datasheets

Certificate of Analysis