Chicken Anti-Actin (Nitrocellulose Membrane Loading Control)
Note that this product does not work in transfers using PVDF membranes.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent
Release criteria
Protein Concentration:
10 mg/mL
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | Actin purified from rabbit muscle. |
| Immunogen Species: | Rabbit |
| Species Reactivity: | Human, Mouse, Rat |
| Molecular Weight: | 42 kDa |
| RRID: | AB_2313504 |
| Purification: | Mixture of IgY fraction and affinity-purified antibodies |
| Physical State: | Liquid |
| Buffer: | Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent |
| Protein Concentration: | 10 mg/mL |
| Application Notes: | Recommended starting dilution: WB Brain 1:1000 – 1:2000. |
| Testing: | This anti-Actin IgY preparation was analyzed by Western Blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat. # H-1004) as the secondary reagent. |
| Storage Conditions: | -20°C |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt. |
Product Applications
- Western blot