Mouse Anti-Tubulin, Beta III

Protein G purified mouse monoclonal antibody to mouse and rat Tubulin, beta III.

Mouse Anti-Tubulin, Beta III
Mouse Anti-Tubulin, Beta III
Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine.
Immunostaining of mouse dentate gyrus showing beta III tubulin (red, 1:1000). The blue is staining nuclei. Photo courtesy of Robert Wine.
Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~55 kDa beta III tubulin protein.
Immunolabelling of the CA3 subfield of mouse hippocampus labeling β-III tubulin 1:1000, red) and CNP green, 1:500). The blue is DAPI staining DNA. Photo courtesy of Rob Wine.
Immunolabelling of the CA3 subfield of mouse hippocampus labeling β-III tubulin 1:1000, red) and CNP green, 1:500). The blue is DAPI staining DNA. Photo courtesy of Rob Wine.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
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Product code: AMSTU3

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Unit priceFrom $164.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: AA10
Isotype: IgG
Immunogen: Synthetic peptide from residues specific to rat beta III tubulin, conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Species: Rat
Species Reactivity: Rat
Gene Name: TUBB3
Molecular Weight: 55 kDa
RRID: AB_2492275
Purification: Protein G-purified from culture supernatant
Physical State: Liquid
Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per mL BSA, 50% glycerol
Specificity: Recognizes endogenous ~55 kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1.
Application Notes: Recommended starting dilutions: WB 1:10000; WB Brain 1:10000; IHC 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
Documents & Datasheets

Certificate of Analysis