Mouse Anti-Trop-2/TACSTD2 (Extracellular Region)

Protein G purified mouse monoclonal antibody to human Trop-2/TACSTD2 (Extracellular region).

Mouse Anti-Trop-2/TACSTD2 (Extracellular region)
Mouse Anti-Trop-2/TACSTD2 (Extracellular region)
Immunocytochemical labeling of Trop-2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Trop-2 (clone M005). The antibody was detected using goat anti-mouse DyLight® 594.
Immunocytochemical labeling of Trop-2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Trop-2 (clone M005). The antibody was detected using goat anti-mouse DyLight® 594.
Representative Standard Curve using mouse monoclonal anti-Trop-2 for ELISA capture of human recombinant Trop-2 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Representative Standard Curve using mouse monoclonal anti-Trop-2 for ELISA capture of human recombinant Trop-2 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Western blotting for selected HGSC‐specific EV‐associated proteins on U fractions of 11 patients and Caov‐3 and Kuramochi HGSC cell lines. Caov‐3 and Kuramochi cells are used as negative controls for macrophage markers MRC1, CD68, and FCGR1A. For TACSTD2 (1:500), Caov‐3 serves as a positive control, while Kuramochi serves as a negative control. Image from publication CC-BY-4.0. PMID: 38490958
Western blotting for selected HGSC‐specific EV‐associated proteins on U fractions of 11 patients and Caov‐3 and Kuramochi HGSC cell lines. Caov‐3 and Kuramochi cells are used as negative controls for macrophage markers MRC1, CD68, and FCGR1A. For TACSTD2 (1:500), Caov‐3 serves as a positive control, while Kuramochi serves as a negative control. Image from publication CC-BY-4.0. PMID: 38490958

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSTTEG1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M005
Isotype: IgG1
Immunogen: Clone M005 was generated from a proprietary antigen related to the extracellular region of human Trop-2 from the A431 epidermoid carcinoma cell line.
Immunogen Species: Human
Species Reactivity: Human
Gene Name: TACSTD2
Molecular Weight: 50-65
Purification: Protein G-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Clone M005 detects a 50-65 kDa* band matching the molecular mass of Trop-2 on Western blots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured Trop-2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:100; IP 1:100.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis