Mouse Anti-Talin (ROD domain)

Protein A purified mouse monoclonal antibody to chicken, human, mouse, rat and Xenopus Talin (Rod domain).

Mouse Anti-Talin (ROD domain)
Mouse Anti-Talin (ROD domain)
Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.
Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.
Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody, then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).
Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody, then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSTT1G1-100UL

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Unit price$292.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 8D4
Isotype: IgG1
Immunogen: Clone 8D4 was generated from purified chicken gizzard talin. The antibody recognizes an epitope within the rod domain of talin from many species, including human, rat, mouse, chicken, and frog.
Immunogen Species: Chicken
Species Reactivity: Chicken, Frog, Human, Mouse, Rat
Gene Name: TLN1
Molecular Weight: 240
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 240 kDa* protein matching the molecular mass of Talin on Western blots of human A431, rat PC12, and rabbit fibroblast cells.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:100; IP 1:100.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Cancer research
Documents & Datasheets

Certificate of Analysis