Mouse Anti-Spectrin Alpha Chain, Non-Erythrocytic 1
Affinity purified mouse monoclonal antibody raised against Spectrin Alpha Chain.
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG1.
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | 3D7 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (residues 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in and purified from E. coli. |
| Immunogen Species: | Human |
| Species Reactivity: | Bovine, Human, Mouse, Pig, Rat |
| Molecular Weight: | 240 kDa |
| Purification: | Affinity-purified |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | Human; bovine; porcine; rat;. |
| Application Notes: | Recommended starting dilutions: WB 1:5000 – 1:10000; IHC 1:1000 – 1:2000; ICC 1:1000 – 1:2000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)