Mouse Anti-Phosphoserine/Threonine

Protein A purified mouse monoclonal antibody to human, mouse and rat Anti-Phosphoserine/threonine.

Mouse Anti-Phosphoserine/Threonine
Mouse Anti-Phosphoserine/Threonine
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1 and 2) then treated with lambda phosphatase (lane 3). The blot was probed with anti-Phosphoserine/threonine mouse monoclonal at 1:250 (lane 1) or 1:1000 (lanes 2 & 3).
Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine and rabbit polyclonal anti-Phosphoserine/threonine, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Immunocytochemical labeling of phosphoserine and phosphothreonine in control and calyculin A-treated A431 cells. The cells were labeled with mouse monoclonal anti-Phosphoserine/threonine and rabbit polyclonal anti-Phosphoserine/threonine, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Western blot showing phosphorylation level (1:2000) of NFATC1 in TAZ overexpressed HEK-293T cells after 1 mM Tg treatment or UCHL1 knockout. Image from publication CC-BY-4.0. PMID: 37215988
Western blot showing phosphorylation level (1:2000) of NFATC1 in TAZ overexpressed HEK-293T cells after 1 mM Tg treatment or UCHL1 knockout. Image from publication CC-BY-4.0. PMID: 37215988
WT and pda1Δ cells expressing Qcr2-GFP were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. The immunoprecipitates were then probed with α- phosphoamino acid antibody (top panel) and α-GFP antibody (bottom panel). Right panel: day 5 samples were treated overnight with lambda phosphatase or mock-treated. Image from publication CC-BY-4.0. PMID: 37454238
WT and pda1Δ cells expressing Qcr2-GFP were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. The immunoprecipitates were then probed with α- phosphoamino acid antibody (top panel) and α-GFP antibody (bottom panel). Right panel: day 5 samples were treated overnight with lambda phosphatase or mock-treated. Image from publication CC-BY-4.0. PMID: 37454238
WT and pda1Δ cells expressing Mdh1-GFP, as well as the indicated plasmids, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody, and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector Image from publication CC-BY-4.0. PMID: 3745423
WT and pda1Δ cells expressing Mdh1-GFP, as well as the indicated plasmids, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody, and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector Image from publication CC-BY-4.0. PMID: 3745423
Mutant lat1Δ cells expressing the indicated plasmids, as well as Mdh1-GFP, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody, and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector. Image from publication CC-BY-4.0. PMID: 37454238
Mutant lat1Δ cells expressing the indicated plasmids, as well as Mdh1-GFP, were grown in SL medium for the indicated amounts of time, and protein extracts were generated and immunoprecipitated with with α-GFP antibody. Phosphorylation of Mdh1-GFP was detected using α-phosphoamino acid antibody, and total GFP signal in the immunoprecipitate was observed by immunoblotting with the α-GFP antibody. ϕ denotes an empty vector. Image from publication CC-BY-4.0. PMID: 37454238
In vitro kinase assay of nrdD stained with a serine/threonine site-specific antibody . Image from publication CC-BY-4.0. PMID: 38683735
In vitro kinase assay of nrdD stained with a serine/threonine site-specific antibody . Image from publication CC-BY-4.0. PMID: 38683735

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Learn more

Product code: AMSPHTG1-100UL

Custom/Bulk request
Unit price$348.00
Add to Quote

Lead time on this item is currently: 2-4 Weeks

Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426

Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M380A/M380B
Isotype: IgG1
Immunogen: PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (conjugated to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (conjugated to carrier protein).
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
Application Notes: Recommended starting dilutions: WB 1:500; ICC 1:50; IP 1:50.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
  • Cancer research
Documents & Datasheets

Certificate of Analysis