Mouse Anti-P-ERK1/2 (MAPK, Thr-202/Tyr-204)
Protein A purified mouse monoclonal antibody to human, mouse and rat ERK1/2 (Thr-202/Tyr-204).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | M206 |
| Isotype: | IgG1 |
| Immunogen: | Clone M206 was generated from a dual phosphorylated ERK1 (Thr-202/Tyr-204) synthetic peptide (conjugated to carrier protein) matching residues around Thr-202 and Tyr-204 in human ERK1. This sequence is conserved in rat and mouse ERK1, and is highly conserved in ERK2. |
| Immunogen Species: | Human |
| Species Reactivity: | Human, Mouse, Rat |
| Gene Name: | MAPK1 |
| Molecular Weight: | 42/44 |
| Purification: | Protein A-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol |
| Specificity: | Detects 42 and 44 kDa* proteins matching ERK1 and ERK2 on Western blots of human A431 epithelial cells stimulated with EGF or calyculin A. It does not detect bands in control cells. |
| Application Notes: | Recommended starting dilutions: WB 1:1000; ICC 1:100. |
| Testing: | Western blot on every lot. |
| Storage Conditions: | -20°C |
| Handling: | Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for at least 1 year from receipt when stored at -20°C. |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Cancer research