Mouse Anti-P-EGFR (Tyr1101)

Protein A purified mouse monoclonal antibody to human, mouse and rat EGFR (Tyr-1101).

Mouse Anti-P-EGFR (Tyr1101)
Mouse Anti-P-EGFR (Tyr1101)
Western blot image of human A431 cells untreated (lanes 1 & 3) or treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-EGFR (Tyr-1101) monoclonal antibody (lanes 1 & 2) or anti-EGFR (Cytoplasmic) monoclonal antibody (lanes 3 & 4).
Western blot image of human A431 cells untreated (lanes 1 & 3) or treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-EGFR (Tyr-1101) monoclonal antibody (lanes 1 & 2) or anti-EGFR (Cytoplasmic) monoclonal antibody (lanes 3 & 4).
Immunocytochemical labeling in A431 cells untreated or treated with EGF (100 ng/ml) for 5 min. The cells were labeled with mouse monoclonals anti-EGFR and anti-EGFR (Tyr-1101).
Immunocytochemical labeling in A431 cells untreated or treated with EGF (100 ng/ml) for 5 min. The cells were labeled with mouse monoclonals anti-EGFR and anti-EGFR (Tyr-1101).

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSEG8G1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M199
Isotype: IgG1
Immunogen: Clone M199 was generated from a phospho-EGFR (Tyr-1101) synthetic peptide (conjugated to KLH) matching residues around tyrosine 1101 of human EGFR (ErbB-1). This human EGFR sequence has high homology to rat and mouse EGFR, and is not conserved in other ErbB family members. This site is Tyr-1125 in the P00533-1 version of the human EGFR sequence.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: EGFR
Molecular Weight: 180
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: This antibody antibody detects a 180 kDa* protein on Western blots of human A431 cells treated with EGF, and does not detect this band in control cells.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:200.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Cancer research
Documents & Datasheets

Certificate of Analysis