Mouse Anti-Neurofilament NF-M

Protein G purified mouse monoclonal antibody to bovine, chicken, human, mouse and rat Neurofilament NF-M.

Mouse Anti-Neurofilament NF-M
Mouse Anti-Neurofilament NF-M
Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~145 kDa NF-M protein.
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M 1:5000, red) and anti-Neurofilament-H 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
Immunofluorescence of a section of adult rat frontal cortex labeled with anti-Neurofilament-M 1:5000, red) and anti-Neurofilament-H 1:5000, green). The anti-NFM specifically labels neuron cell bodies and dendrites of pyramidal neurons, while the anti-NFH labels the network of neuronal axons only. The blue is Hoechst staining of nuclear DNA.
Immunostaining of cultured rat neurons showing labeling of NF-M green, 1:100) in mature neurons.
Immunostaining of cultured rat neurons showing labeling of NF-M green, 1:100) in mature neurons.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
Learn more

Product code: AMSNFMG1

Custom/Bulk request
Unit priceFrom $154.00
Add to Quote

Lead time on this item is currently: 2-4 Weeks

Need help? Ask a Specialist Contact UsOR +1 (800) 643-3426

Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 3H11
Isotype: IgG1
Immunogen: Preparation containing the extreme C-terminal region of rat NF-M, expressed in and purified from E. coli.
Immunogen Species: Rat
Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Gene Name: NEFM
Molecular Weight: 145 kDa
RRID: AB_2492163
Purification: Protein G-purified
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~145 kDa neurofilament M protein.
Application Notes: Recommended starting dilutions: WB 1:1000; WB Brain 1:1000; IHC 1:500 – 1:5000; ICC 1:100 – 1:500.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis