Mouse Anti-Neurofilament NF-L

Protein G purified mouse monoclonal antibody to bovine, chicken, human, mouse and rat Neurofilament NF-L.

Mouse Anti-Neurofilament NF-L
Mouse Anti-Neurofilament NF-L
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody green, 1:100) and anti-alpha internexin.
Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody green, 1:100) and anti-alpha internexin.
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody red, 1:25,000) and anti-NFL antibody green, 1:100).
Immunostaining of mixed cultured rat neurons and glia stained with anti-NFH antibody red, 1:25,000) and anti-NFL antibody green, 1:100).
Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L 1:500, red) and labeling of GFAP 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.
Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L 1:500, red) and labeling of GFAP 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: AMSNEFG1

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: DA2
Isotype: IgG1
Immunogen: Preparation of porcine NF-L.
Immunogen Species: Pig
Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Gene Name: NEFL
Molecular Weight: 68 kDa
RRID: AB_2492161
Purification: Protein G-purified
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~68 kDa Neurofilament L protein.
Application Notes: Recommended starting dilutions: WB 1:1000; WB Brain 1:1000; IHC 1:100 – 1:1000; ICC 1:100 – 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis