Mouse Anti-Neurofilament NF-H

Protein G purified mouse monoclonal antibody to all known mammal and chicken Neurofilament NF-H.

Mouse Anti-Neurofilament NF-H
Mouse Anti-Neurofilament NF-H
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
Immunostaining of human cerebellar cortex showing labeling of NF-H DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.
Immunostaining of human cerebellar cortex showing labeling of NF-H DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.

Specifications

Physical State:
Liquid
Storage:
-20°C or -80°C (long term), 2 – 8°C (short term)
Buffer:
PBS, 10 mM sodium azide
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Product code: AMSNFHG1

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Unit priceFrom $154.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: NAP4
Isotype: IgG1
Immunogen: Purified bovine NF-H
Immunogen Species: Bovine
Species Reactivity: Chicken
Gene Name: NEFH
Molecular Weight: 200 kDa
RRID: AB_2492159
Purification: Protein G-purified
Physical State: Liquid
Buffer: PBS, 10 mM sodium azide
Specificity: Recognizes endogenous ~200 kDa Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
Application Notes: Recommended starting dilutions: WB 1:5000; WB Brain 1:5000; IHC 1:500 – 1:1000; ICC 1:500 – 1:1000.
Testing: Western blot on every lot.
Storage Conditions: -20°C or -80°C (long term), 2 – 8°C (short term)
Handling: Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis