Mouse Anti-Neurofilament Light Polypeptide (NF-L, DA2)
Affinity purified mouse monoclonal antibody raised against Neurofilament Light Polypeptide (NF-L, DA2).
Specifications
Physical State:
Liquid
Storage:
-20°C (long term), 2 – 8°C (short term)
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG1.
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | DA2 |
| Isotype: | IgG1 |
| Immunogen: | Raised against enzymatically dephosphorylated, full-length, pig NF-L protein. The antibody binding epitope has been mapped to a short peptide in the C-terminal “tail†region of the molecule within the sequence YYTSHVQEEQIEVEETIEA, residues 441-460 of the human sequence. |
| Immunogen Species: | Pig |
| Species Reactivity: | Bovine, Horse, Human, Mouse, Pig, Rat |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | Species cross-reactivity includes human, rat, mouse, cow, pig and horse. |
| Application Notes: | Recommended starting dilutions: WB 1:5000; IHC 1:2000; ICC 1:1000. |
| Storage Conditions: | -20°C (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research