Mouse Anti-Na+/K+ ATPase β3 (Extracellular)

Protein G purified mouse monoclonal antibody to human Na+/K+ ATPase β3 (Extracellular).

Mouse Anti-Na+/K+ ATPase β3 (Extracellular)
Mouse Anti-Na+/K+ ATPase β3 (Extracellular)
Western blot of immunoprecipitates (IP) and whole lysates. The antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-Na+/K+ ATPase β3 (lanes 1-7). The arrow designates native antibody, while the β3 subunit migrates around 40 kDa.
Western blot of immunoprecipitates (IP) and whole lysates. The antibody only (lane 1), IP from A431 cell lysate (lane 2), A431 cell input (lane 3), LNCaP cells (lane 4), MeWo cells (lane 5), and normal human lung (lane 6) and skin (lane 7). The blot was probed with anti-Na+/K+ ATPase β3 (lanes 1-7). The arrow designates native antibody, while the β3 subunit migrates around 40 kDa.
Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight® 594.
Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M016). The antibody was detected using goat anti-mouse Ig DyLight® 594.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSNKAG1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M016
Isotype: IgG1
Immunogen: Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in A431 epidermoid carcinoma cell line.
Immunogen Species: Human
Species Reactivity: Human
Gene Name: ATP1B3
Molecular Weight: 40
Purification: Protein G-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase β3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:50; IP 1:50.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Immunoprecipitation
Documents & Datasheets

Certificate of Analysis