Mouse Anti-Myelin Basic Protein (MBP) (Clone 7D2)
Mouse monoclonal antibody raised against a preparation of MBP purified biochemically from bovine brain.
Specifications
Lead time on this item is currently: 2-4 Weeks
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Product Overview
It can be used to identify oligodendrocytes and Schwann cells in cell culture, to visualize myelin sheaths and myelinating cells in sectioned material and to probe western blots for MBP gene products. The antibody is also rather insensitive to aldehyde fixation and so can be used in immunohistochemistry of paraffin sections and in CLARITY type applications. The MCA-7D2 antibody binds only the 21.5kDa and 18.5kDa rat MBP isotypes, but all four isotypes of human and bovine MBP, mapping the epitope to amino acids 145-184 of the human 21.5kDa sequence. The antibody works well for western blotting and for IF, ICC and IHC. A sequence alignment of the four CNS MBP isotypes in human and rat can be downloaded from here.
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | 7G7 |
| Isotype: | IgG |
| Immunogen: | Three peptide sequences conserved in higher verterbrate MBP protein. |
| Immunogen Species: | Bovine |
| Species Reactivity: | Bovine, Human, Rat |
| Molecular Weight: | 21.5 kDa and 18.5 kDa |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | The specificity of this antibody has been confirmed by WB. This antibody stains bands around 21.5 kDa and 18.5 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Human, rat. |
| Application Notes: | Recommended starting dilutions: WB 1:5000 – 1:10000; IHC 1:500 – 1:1000; ICC 1:500 – 1:1000. |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Neuroscience research
- Cell culture