Mouse Anti-Microtubule Associated Protein 2 C/D (MAP2C/D)

Protein G purified mouse monoclonal antibody to bovine, human, mouse and rat Microtubule Associated Protein 2 C/D (MAP2C/D).

Mouse Anti-Microtubule Associated Protein 2 C/D (MAP2C/D)
Mouse Anti-Microtubule Associated Protein 2 C/D (MAP2C/D)
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70 kDa MAP2C/D proteins and the ~280 kDa MAP2A/B proteins.
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~70 kDa MAP2C/D proteins and the ~280 kDa MAP2A/B proteins.
Immunofluorescence of a section of adult rat hippocampus section stained with Anti-MAP2C green, 1:5,000) and an anti-FOX2 antibody (red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The anti-MAP2C labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites.
Immunofluorescence of a section of adult rat hippocampus section stained with Anti-MAP2C green, 1:5,000) and an anti-FOX2 antibody (red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The anti-MAP2C labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites.
Immunostaining of mixed neuron and glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells with anti-microtubule associated protein 2C/D red, 1:2500) and specific fibroblast, astrocyte labeling with anti-vimentin green, 1:500). The blue stain is DAPI to identify nuclei.
Immunostaining of mixed neuron and glial cultures showing specific cytoplasmic labeling of dendrites and perikarya of neuronal cells with anti-microtubule associated protein 2C/D red, 1:2500) and specific fibroblast, astrocyte labeling with anti-vimentin green, 1:500). The blue stain is DAPI to identify nuclei.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 50% glycerol, 5 mM sodium azide
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Product code: AMSMA2G2

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Unit priceFrom $164.00
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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: 2C4
Isotype: IgG2a
Immunogen: Full length recombinant human MAP2D protein.
Immunogen Species: Human
Species Reactivity: Bovine, Human, Mouse, Rat
Gene Name: MAP2
Molecular Weight: 70 kDa
RRID: AB_2571638
Purification: Protein G-purified from culture supernatant
Physical State: Liquid
Buffer: PBS, 50% glycerol, 5 mM sodium azide
Specificity: Recognizes endogenous ~70 kDa MAP2C/D isoforms as well as the ~280 kDa MAP2A/B. The antibody will bind all known MAP2 isoforms.
Application Notes: Recommended starting dilutions: WB 1:5000; WB Brain 1:5000; IHC 1:2500 – 1:10,000; ICC 1:2500 – 1:10,000.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunohistochemistry
  • Immunofluorescence (IF/ICC)
  • Neuroscience research
Documents & Datasheets

Certificate of Analysis