Mouse Anti-mCherry (Clone 1C51)
Mouse monoclonal antibody raised against full length recombinant mCherry expressed in and purified from E. coli.
Specifications
Physical State:
Liquid
Storage:
-20°C Short term, -80°C Long term
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
The antibody recognizes mCherry strongly on western blots, in appropriate cells and sections and does not react with GFP. It does however react equally well with tdTomato, another derivative of DsRed, see data under the "Additional Data" tab. It can be used to verify the size of fusion constructs by western blotting and to amplify the endogenous fluorescence of mCherry in cells and tissues, including formalin fixed paraffin embedded sections, see additional info tab. Clone 1C51.
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | 1C51 |
| Isotype: | IgG2a |
| Immunogen: | Recombinant full length mCherry expressed and purified from E. coli. |
| Immunogen Species: | Species Independent |
| Molecular Weight: | 28 kDa |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | The antibody reacts with a band at ~28 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry. |
| Application Notes: | WB: 1:1000-1:2000 ICC: 1:250-1:500 |
| Storage Conditions: | -20°C Short term, -80°C Long term |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
