Chicken Anti-GFP (Recombinant Chimeric FL490 Conjugate)
Chicken monoclonal antibody to GFP, purified by affinity chromatography.
Specifications
Physical State:
Liquid
Storage:
-20°C or below (long term), 2 – 8°C (short term)
Buffer:
PBS, 0.05% Sodium Azide pH 7.4
Release criteria
Protein Concentration:
0.3 – 0.5 mg/mL (lot dependent)
Lead time on this item is currently: 2-4 Weeks
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Product Overview
| Host Species: | Chicken |
|---|---|
| Clonality: | Recombinant Monoclonal |
| Clone: | N86/38 |
| Isotype: | IgY |
| Conjugate: | FL490 (Ex 491 nm / Em 515 nm) |
| Immunogen: | Recombinant fusion protein spanning residues 1-238 (full-length) of jellyfish green fluorescent protein (UniProt P42212), expressed in E. coli. |
| Immunogen Species: | Jellyfish |
| Gene Name: | GFP |
| Molecular Weight: | 27 kDa |
| Purification: | Affinity-purified |
| Physical State: | Liquid |
| Buffer: | PBS, 0.05% Sodium Azide pH 7.4 |
| Protein Concentration: | 0.3 – 0.5 mg/mL (lot dependent) |
| Specificity: | Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed). |
| Application Notes: | Recommended starting dilutions: IHC 1:500 – 1:1000; ICC 1:500 – 1:1000. |
| Testing: | Each lot is QC-tested on cells overexpressing target protein for the expected staining pattern. |
| Storage Conditions: | -20°C or below (long term), 2 – 8°C (short term) |
| Handling: | Aliquot on receipt. Keep a working aliquot at 2 – 8°C for short-term use. Spin the vial down before opening. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 24 months from receipt. |
Product Applications
- Western blot
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
