Chicken Anti-Beta-Gal (Beta-Galactosidase, LacZ)
Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity.
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with thimerosal (0.01%,w/v) as an anti-microbial agent
Release criteria
Protein Concentration:
10 mg/mL
Lead time on this item is currently: 2-4 Weeks
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Product Overview
It even works well with fusion proteins of LacZ!
| Host Species: | Chicken |
|---|---|
| Clonality: | Polyclonal |
| Isotype: | IgY |
| Immunogen: | Native protein expressed Escherichia coli. |
| Molecular Weight: | 117 kDa |
| RRID: | AB_2313508 |
| Purification: | Mixture of IgY fraction and affinity-purified antibodies |
| Physical State: | Liquid |
| Buffer: | Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with thimerosal (0.01%,w/v) as an anti-microbial agent |
| Protein Concentration: | 10 mg/mL |
| Application Notes: | Recommended starting dilutions: WB 1:5000 – 1:10000; IHC 1:2500 – 1:5000; ICC 1:2500 – 1:5000. |
| Testing: | This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005). |
| Storage Conditions: | -20°C |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt. |
Product Applications
- Western blot
- ELISA
- Immunohistochemistry
- Immunofluorescence (IF/ICC)
- Enzyme conjugation
