Mouse Anti-Integrin β4 (Cytoplasmic Region)

Protein A purified mouse monoclonal antibody to human Integrin β4 (Cytoplasmic region).

Mouse Anti-Integrin β4 (Cytoplasmic region)
Mouse Anti-Integrin β4 (Cytoplasmic region)
Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-Integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin β4 (lanes 5 & 6).
Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin β4 (Tyr-1526) (lanes 1 & 2) and anti-Integrin β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin β4 (lanes 5 & 6).
Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.
Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSIT4G1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M126
Isotype: IgG1
Immunogen: Clone M126 was generated from a recombinant protein containing residues in the cytoplasmic region of human Integrin β4. This sequence is found in all three Integrin β4 isoforms and has 90% homology with rat and mouse Integrin β4.
Immunogen Species: Human
Species Reactivity: Human
Gene Name: ITGB4
Molecular Weight: 200
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 200kDa* protein matching the molecular mass of Integrin β4 on Western blots of human A431 cells.
Application Notes: Recommended starting dilutions: WB 1:1000; ICC 1:250.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
  • Cancer research
Documents & Datasheets

Certificate of Analysis