Mouse Anti-High-Mobility Group Protein 1 (HMGB1)
Affinity purified mouse monoclonal antibody raised against High-Mobility Group Protein 1 (HMGB1).
Specifications
Physical State:
Liquid
Storage:
-20°C
Buffer:
Learn more Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide
Lead time on this item is currently: 2-4 Weeks
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Product Overview
Isotype IgG2b.
| Host Species: | Mouse |
|---|---|
| Clonality: | Monoclonal |
| Clone: | 1F3 |
| Isotype: | IgG2b |
| Immunogen: | Human full-length recombinant human HMGB1 protein expressed in and purified from E. coli. |
| Immunogen Species: | Human |
| Species Reactivity: | Bovine, Human, Mouse, Pig, Rat |
| Molecular Weight: | 25 kDa |
| Purification: | IgG |
| Physical State: | Liquid |
| Buffer: | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, sodium azide |
| Specificity: | Reacts with a band at ~25 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry showing strong nuclear staining. |
| Application Notes: | Recommended starting dilutions: WB 1:1000 – 1:2000; ICC 1:500 – 1:1000. |
| Storage Conditions: | -20°C |
| Handling: | Aliquot on receipt to avoid repeated freeze-thaw cycles. Spin the vial down before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration before use. |
| Shipping Conditions: | Dry ice |
| Expiration: | Stable for 12 months from receipt (unopened). |
Product Applications
- Western blot
- Immunofluorescence (IF/ICC)
- Neuroscience research