Mouse Anti-GM130 C-Terminus

Protein A purified mouse monoclonal antibody to human, mouse and rat GM130 (C-terminal region).

Mouse Anti-GM130 C-Terminus
Mouse Anti-GM130 C-Terminus
Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.
Immunocytochemical labeling of GM130 in C2C12 cells. The cells were double-labeled with GM130 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.
Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.
Western blot analysis of GM130 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130 (C-terminal region) at 1:500.

Specifications

Physical State:
Liquid
Storage:
-20°C
Buffer:
PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
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Product code: AMSG33G1-100UL

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Unit price$379.00
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Lead time on this item is currently: 2-4 Weeks

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Product Overview

Host Species: Mouse
Clonality: Monoclonal
Clone: M342
Isotype: IgG1
Immunogen: Clone M342 was generated from a recombinant protein matching residues in the C-terminal region of human GM130. This sequence has high homology to similar regions in rat and mouse GM130.
Immunogen Species: Human
Species Reactivity: Human, Mouse, Rat
Gene Name: GOLGA2
Molecular Weight: 130
Purification: Protein A-purified
Physical State: Liquid
Buffer: PBS, 1 mg/mL BSA, 0.05% sodium azide, 50% glycerol
Specificity: Detects a 130 kDa* protein matching the apparent molecular mass of GM130 on Western blots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.
Application Notes: Recommended starting dilutions: WB 1:500; ICC 1:50.
Testing: Western blot on every lot.
Storage Conditions: -20°C
Handling: Supplied in 50% glycerol, so aliquots can be drawn at -20°C without thawing the stock.
Shipping Conditions: Dry ice
Expiration: Stable for at least 1 year from receipt when stored at -20°C.

Product Applications

  • Western blot
  • Immunofluorescence (IF/ICC)
Documents & Datasheets

Certificate of Analysis